Related topics: Alphabetical list, Drosophila, Signal transduction
See list of additional Phospho-Tyrosine antibodies at Linscott’s Directory
On November 5, 2020
Tomoki Ishibashi wrote:
I have used this antibody for IHC on Drosophila embryo. Dilution: 1:1000
On November 28, 2017
Anders Dalby wrote:
I have used this antibody for both WB and IP and it works very well in both categories.
Phosphorus-32 labelled primary murine neurons were lysed and by using 1ug/mL i was able to IP and detect clear bands corresponding to tyrosine-phosphorylated proteins.
For WB i use 1:1000 dilution. Works well but with little background.
On February 9, 2014
Simon Glerup, Aarhus University wrote:
We stimulated SY5Y neuroblastoma cells transfected with human TrkB with increasing concentrations of BDNF for 15 min. Cells were lysed in TNE buffer containing protease and phosphatase inhibitors. Lysates were analyzed by Western blotting and blots were developed using ECL Plus.
We observed a dose-dependent response to BDNF using this antibody and several bands became visible upon BDNF stimulation compared to unstimulated cells and untransfected cells.
WB dilution 1:1000
On October 8, 2013
Yuval Yarkoni wrote:
I have used 4G10 from Millipore (http://www.millipore.com/catalogue/item/05-321)
for WB at 0.1ug/ml on lysates from B cell lymphomas and it worked great.
I have used a home-made conjugate of 4G10 to Alexa 488 for intracellular flow. Since there is allways phosphorylated Tyr proteins in cells, it served as a positive control for my intracellular staining. Worked great.